Wow, I didn't even think of the fact that muscle cells are being used to express the spike and what factor that would play, but given your previous post on the HeLa contamination this should raise a lot of questions. Now I'm wondering if the multinucleated nature of muscle cells may actually be an issue in both gene expression and uptake of even things such as the adenoviral vectors. Lots more things to ponder and it raises even more questions! When it comes to glycosylation do you suppose that the cell lines used for studies would be critical as well, such that in vitro assays may not provide a viable examination of the true glycosylation occurring if we assume that there will be high variability due to different biochemical processes.
But I suppose then that with those with severe COVID and widespread viral infection should we assume differences in protein conformation and glycosylation? Sorry This whole post has made me really curious about everything!
The fact that the actual spike protein from the vaccine hasn't been fully elucidated is rather alarming as well. Do you know how the mRNA is constructed Joomi? I generally assumed some sort of chip technology automated with some base insertion/wash sequence, but I suppose the type of method would also raise questions as to the fragments produced and whether they were properly isolated.
What the hell? How could regulators not have asked if the proteins churned out were actually what was expected? Was no sequencing done at all? Or just dodgy blots?
I do not know how anybody could trust a industry that has complete liability protection(a license to kill or maim). It appears they set out under the guise of creating a vaccine, a way to create more customers for there drugs, and with all the data they created a maze to keep people looking for god knows what as a explanation for whats going on they knew this is all part of a sick business model that has been ongoing since the Flexner report that changed how medical schools teach.
Joomi’s explanations of the jabs’ unknowns are clear and relatively easy to understand. Why haven’t science journalists reported on this critical matter?
(No need to answer. There are no uncaptured science journalists working for news outlets.)
An analogy for how the mRNA technology is supposed to work: It is like tossing pieces of a violin into a concert hall and expecting the orchestra to play your chosen tunes.
What an absolutely stellar article! Thank you, thank you for clarifying the science and tech used in these studies for those of us who aren't biologists or chemists.
Sep 29, 2022·edited Sep 29, 2022Liked by Joomi Kim
Thanks for another exceptionally clearly written article. Since you previously wrote about spike infiltration into the nucleus, you will be interested in this preprint.
The upshot being that the researchers in this new paper believe a sequence including the infamous furin cleavage site allows nucleus entry. They found mRNA co-located with spike in the nucleus and never mRNA in the nucleus that was not co-located.
In the context of what is discussed here, what does it mean if mRNA produces spike that moves mRNA back into the nucleus? Does that cause multiple cycles of this splicing? Sounds bad.
Some short peptide fragments of SARS-CoV-2 Spike are amyloidogenic. Others have superantigenic regions, regions that bind bacterial endotoxins, and others that look very similar to snake venom neurotoxin.
People on Twitter are all like "But SIMOA assays show an infinitesimally small amount of cleaved S1 in the bloodstream!"
Yes. Assays that are specifically designed to find whole Spike or its intact subunits. Not bits of it cleaved apart by host enzymes, nor mistranslated polypeptide fragments, which, given the very low purity of the mRNA in these formulations, is almost a certainty.
By the way, did Katalin Kariko even check to see if pseudouridylated and synthetically capped mRNA evaded detection by TLR7/8, or if it actually behaved as a TLR7/8 inhibitor?
Thanks for this post. I never really thought the MRNA would produce a variety of proteins folded in different ways, but of course it is a possibility. You do a good job making complex things understandable. 👍🏽👏
Wow, I didn't even think of the fact that muscle cells are being used to express the spike and what factor that would play, but given your previous post on the HeLa contamination this should raise a lot of questions. Now I'm wondering if the multinucleated nature of muscle cells may actually be an issue in both gene expression and uptake of even things such as the adenoviral vectors. Lots more things to ponder and it raises even more questions! When it comes to glycosylation do you suppose that the cell lines used for studies would be critical as well, such that in vitro assays may not provide a viable examination of the true glycosylation occurring if we assume that there will be high variability due to different biochemical processes.
But I suppose then that with those with severe COVID and widespread viral infection should we assume differences in protein conformation and glycosylation? Sorry This whole post has made me really curious about everything!
The fact that the actual spike protein from the vaccine hasn't been fully elucidated is rather alarming as well. Do you know how the mRNA is constructed Joomi? I generally assumed some sort of chip technology automated with some base insertion/wash sequence, but I suppose the type of method would also raise questions as to the fragments produced and whether they were properly isolated.
What the hell? How could regulators not have asked if the proteins churned out were actually what was expected? Was no sequencing done at all? Or just dodgy blots?
I do not know how anybody could trust a industry that has complete liability protection(a license to kill or maim). It appears they set out under the guise of creating a vaccine, a way to create more customers for there drugs, and with all the data they created a maze to keep people looking for god knows what as a explanation for whats going on they knew this is all part of a sick business model that has been ongoing since the Flexner report that changed how medical schools teach.
Hi Joomi,
I recently discovered your substack via GigaohmBiological. I saw your interview there.
You are doing a fabulous job, thank you for sharing, I'll be paying attention.
Take care,
Agus
Great post. Thanks for doing this.
Joomi’s explanations of the jabs’ unknowns are clear and relatively easy to understand. Why haven’t science journalists reported on this critical matter?
(No need to answer. There are no uncaptured science journalists working for news outlets.)
An analogy for how the mRNA technology is supposed to work: It is like tossing pieces of a violin into a concert hall and expecting the orchestra to play your chosen tunes.
This is a great article putting together a few sources that we have been discussing for a while. Thanks for making it simple for everyone!
What an absolutely stellar article! Thank you, thank you for clarifying the science and tech used in these studies for those of us who aren't biologists or chemists.
Thanks for another exceptionally clearly written article. Since you previously wrote about spike infiltration into the nucleus, you will be interested in this preprint.
https://www.biorxiv.org/content/10.1101/2022.09.27.509633v1.full.pdf
Also discussed here:
https://arkmedic.substack.com/p/new-study-vindicates-jiang-and-mei/comments
The upshot being that the researchers in this new paper believe a sequence including the infamous furin cleavage site allows nucleus entry. They found mRNA co-located with spike in the nucleus and never mRNA in the nucleus that was not co-located.
In the context of what is discussed here, what does it mean if mRNA produces spike that moves mRNA back into the nucleus? Does that cause multiple cycles of this splicing? Sounds bad.
Some short peptide fragments of SARS-CoV-2 Spike are amyloidogenic. Others have superantigenic regions, regions that bind bacterial endotoxins, and others that look very similar to snake venom neurotoxin.
https://pubs.acs.org/doi/10.1021/jacs.2c03925
https://www.nature.com/articles/s41577-021-00502-5
https://academic.oup.com/jmcb/article/12/12/916/6028992
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461543/
People on Twitter are all like "But SIMOA assays show an infinitesimally small amount of cleaved S1 in the bloodstream!"
Yes. Assays that are specifically designed to find whole Spike or its intact subunits. Not bits of it cleaved apart by host enzymes, nor mistranslated polypeptide fragments, which, given the very low purity of the mRNA in these formulations, is almost a certainty.
By the way, did Katalin Kariko even check to see if pseudouridylated and synthetically capped mRNA evaded detection by TLR7/8, or if it actually behaved as a TLR7/8 inhibitor?
https://www.medrxiv.org/content/10.1101/2021.05.03.21256520v1
Big oops.
Good article. Thank you for writing it.
This is bloody brilliant. Here's my write-up from February:
https://jessicar.substack.com/p/evidence-of-connection-between-severe
Thanks for this post. I never really thought the MRNA would produce a variety of proteins folded in different ways, but of course it is a possibility. You do a good job making complex things understandable. 👍🏽👏
EXCELLENT!!!
Brilliant thorough analysis!
I am sharing this around. You do a wonderful job educating the reader. I've bookmarked this post.
THANK YOU!
Thank you!
I am hoping that an interview with J C Couey is contemplated in the very near future!
Great explanation. Thanks.